![]() ![]() ![]() Taken together, these results demonstrated that PbrWRKY53 plays a positive role in drought tolerance, which might be, at least in part, promoting production of vitamin C via regulating PbrNCED1 expression.ĭrought stress is one of the most major environmental limited factors that seriously hampers crop productivity. Yeast one‐hybrid assay, EMSA and transient expression analysis demonstrated that PbrWRKY53 could bind to the W‐box element in the promoter region of PbrNCED1. Knock‐down of PbrWRKY53 in P. ussuriensis down‐regulated PbrNCED1 abundance, accompanied by compromised drought tolerance. In addition, overexpression of PbrWRKY53 in transgenic tobacco resulted in enhanced expression level of PbrNCED1, and led to the increase in larger amount of vitamin C accumulation in comparison to WT. The transgenic plants exhibited better water status, less reactive oxygen species generation and higher levels of antioxidant enzyme activities and metabolites than the wild type. Ectopic expression of PbrWRKY53 in tobacco and Pyrus ussuriensis conferred enhanced tolerance to drought stress. Subcellar localization analyses showed that PbrWRKY53 was located in the nucleus. PbrWRKY53 was greatly up‐regulated by drought and abscisic acid, but slightly induced by salt and cold. In this study, we report the identification and functional characterization of PbrWRKY53 isolated from Pyrus betulaefolia. WRKY comprises a large family of transcription factors in plants, but most WRKY members are still poorly understood. Taken together, these results demonstrated that PbrWRKY53 plays a positive role in drought tolerance, which might be, at least in part, promoting production of vitamin C via regulating PbrNCED1 expression. Yeast one-hybrid assay, EMSA and transient expression analysis demonstrated that PbrWRKY53 could bind to the W-box element in the promoter region of PbrNCED1. Knock-down of PbrWRKY53 in P. ussuriensis down-regulated PbrNCED1 abundance, accompanied by compromised drought tolerance. PbrWRKY53 was greatly up-regulated by drought and abscisic acid, but slightly induced by salt and cold. ![]()
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